ISH NIA PS 02-03 The expression of miR-125a-3p in peripheral blood mononuclear cells of patients with Coronary heart disease

Abstract

Objective: Macrophages play a key role in the occurrence and development of coronary atherosclerosis, tissue macrophages can be divided into two types, the classical activation type (type M1) and alternative activation type (M2 type), M1 type macrophages mainly for promoting inflammation, and M2 type macrophages mainly for anti-inflammatory effect. Recent studies show that the balance of M1/M2 plays an important role in atherosclerotic plaque stability. And a study show that miR-125a-3p plays an important role in the regulation of the differentiation of monocytes to M1 type macrophages. Design and Method: 20 cases of normal control group (group A), and the patients with coronary heart disease were divided into 23 cases of stable angina pectoris group, and 40 cases of acute coronary syndrome group. All people were confirmed by coronary angiography. Extracting object venous blood and separating the mononuclear cells, total RNA was extracted from the resulting mononuclear cells, and then the total RNA was reverse transcribed using the reverse transcription object microRNA reverse primer synthesis, the level of miRNAs in blood was detected by using real-time fluorescent quantitative PCR. To detect the expression level of peripheral blood mononuclear cells differentiation miR-125a-3p in patients of Coronary atherosclerotic heart disease. Results: Compared with group A, the levels of miR-125a-3p in group B’group C were significant high expression (P < 0.01). Compared with group B, the levels of miR-125a-3p in group C was significant high expression (P < 0.01). group C > group B > group A. Conclusions: miR-125a-3p may be new targets for the treatment of unstable plaque.