Abstract
Nanodiscs (NDs) have emerged as an excellent platform for structural and functional characterizations of membrane proteins. We herein further engineered nanodisc as a robust fluorescent sensor to monitor membrane biochemical reactions. Specifically, we circularized nanodiscs via split GFP, and thereby created an intensity-based fluorescent sensor for detecting membrane binding and remodeling events in NDs (isenND). We demonstrated the use of isenND to study the action of several bacterial and eukaryotic membrane proteins on lipids bilayers.
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