Ischemia modified albumin increase indicating cardiac damage after experimental subarachnoid hemorrhage

Şerefden Açıkgöz,Murat Can,İshak Özel Tekin,Figen Barut,Nurullah Edebali,Çağatay Büyükuysal,Bektaş Açıkgöz

doi:10.1186/1471-2202-15-33

Şerefden Açıkgöz, Murat Can + Show 5 more

Open Access

https://doi.org/10.1186/1471-2202-15-33

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Journal: BMC neuroscience	Publication Date: Feb 24, 2014
Citations: 44	License type: CC BY 2.0

Affiliation: Bülent Ecevit University

Abstract

BackgroundCardiac complications are often developed after subarachnoid hemorrhage (SAH) and may cause sudden death of the patient. There are reports in the literature addressing ischemia modified albumin (IMA) as an early and useful marker in the diagnosis of ischemic heart events. The aim of this study is to evaluate serum IMA by using the albumin cobalt binding (ACB) test in the first, second, and seventh days of experimental SAH in rats.Twenty-eight Wistar albino rats were divided into four groups each consisting of seven animals. These were classified as control group, 1st, 2nd and 7th day SAH groups. SAH was done by transclival basilar artery puncture. Blood samples were collected under anesthesia from the left ventricles of the heart using the cardiac puncture method for IMA measurement. Histopathological examinations were performed on the heart and lung tissues. Albumin with by colorimetric, creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) were determined on an automatic analyser using the enzymatic method. IMA using by ACB test was detected with spectrophotometer.ResultsSerum IMA (p = 0.044) in seventh day of SAH were higher compared to the control group. Total injury scores of heart and lung tissue, also myocytolysis at day 7 were significantly higher than control group (p = 0.001, p = 0.001, p = 0.001), day 1 (p = 0.001, p = 0.001, p = 0.001) and day 2 (p = 0.001, p = 0.007, p = 0.001). A positive correlation between IMA - myocytolysis (r = 0.48, p = 0.008), and between IMA – heart tissue total injury score (r = 0.41, p = 0.029) was found.ConclusionThe results revealed that increased serum IMA may be related to myocardial stress after SAH.

Highlights

Cardiac complications are often developed after subarachnoid hemorrhage (SAH) and may cause sudden death of the patient
There are three main theories explaining the pathogenesis of subarachnoid hemorrhage (SAH)-induced cardiac dysfunction [2]
In our study we observed increased levels of ischemia modified albumin (IMA) at days 1 and 2 after SAH, in blood samples obtained by cardiac pucture

Summary

Introduction

Cardiac complications are often developed after subarachnoid hemorrhage (SAH) and may cause sudden death of the patient. The aim of this study is to evaluate serum IMA by using the albumin cobalt binding (ACB) test in the first, second, and seventh days of experimental SAH in rats. Twenty-eight Wistar albino rats were divided into four groups each consisting of seven animals. These were classified as control group, 1st, 2nd and 7th day SAH groups. Blood samples were collected under anesthesia from the left ventricles of the heart using the cardiac puncture method for IMA measurement. There are three main theories explaining the pathogenesis of subarachnoid hemorrhage (SAH)-induced cardiac dysfunction [2].

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