Is there any relationship between the attenuation of cerebral ischemia-reperfusion injury and serum YAP-1 levels?

Abstract

Abstract Objectives: Cerebral ischaemia (CI) is one of the leading causes of death worldwide. Due to the long and costly treatment process after ischemia and the need for lifelong patient care, studies in this field are important. Explaining the mechanism of damage is valuable in terms of developing preventive applications in risky persons and accelerating the treatment processes of sick patients. The aim of this study was to evaluate the relationship between serum yes-associated protein 1 (YAP-1) level, an antiapoptotic marker, and cerebral ischemia-reperfusion (CI/R) injury. Methods: CI was created using the intra-arterial filament technique during 60 minutes, Asprosin (Asp) (1 µg/kg/day) injections were applied 3 days before or 3 days during of 24h and 7 days reperfusion. Wistar Albino adult (10 weeks old, 280-300 g) male rats were randomly assigned to Sham, CI, Asp+CI, and CI+Asp, with 24-hours and 7-day reperfusion periods (n=8). YAP- l levels were measured using customary ELISA kits. The Kruskal-Wallis test was used to compare differences between groups. p<0.05 was considered statistically significant. Results: The decrease in YAP-1 in the CI groups is statistically significant (p<0.05) compared to the sham group. The increase in YAP-1 levels in Asp groups compared with CI group is statistically significant (p<0.05). Conclusions: The serum YAP-1 levels decreased due to CI/R injury. Asp, which is known to be effective in the apoptosis pathway, increased YAP-1 levels when used for protective and therapeutic purposes against CI/R injury. Key words: Brain, Cerebral ischemia, YAP-1, Apoptosis