Abstract
BackgroundThe aim of the present study was to investigate the cardioprotective effect of Taurine on the donor hearts during cold ischemic period.Methods32 rats were divided into four groups (sham, taurine, ischemia, treatment group, 8 rats in each). All rats were fed with rat food for three weeks. Taurine and treatment groups were given a 200 mg/kg/day dose of Taurine by oral gavage besides rat feed. Cardiectomy was performed in all rats after three weeks. In ischemia and treatment groups, harvested hearts were kept in 0.9% sodium chloride at +4 degrees C for 5 hours. Tissue samples were taken from left ventricle in all groups. These samples were evaluated by histopathologic and biochemical examination.ResultsIn the present study results of the biochemical and histopathological examination reveals the protective effects of Taurine. As a marker of lipid peroxidation, Malondialdehyde (MDA) levels in ischemia group were significantly higher than both Sham and Taurine groups. MDA values were recorded; 3.62 ± 0.197 in the sham group, 2.07 ± 0.751 in the Taurine group, 9.71 ± 1.439 in the ischemia group and 7.68 ± 1.365 in the treatment group. MDA levels decreased in treatment group. (p < 0.05) In accordance with MDA findings, while superoxide dismutase and glutathione peroxidase levels decreased in ischemia group, they increased in treatment group. (p < 0.05) There was no differences in Catalase (CAT) enzyme level between treatment and ischemia group (p = 1.000). CAT level results were recorded; 7.08 ± 0.609 in the sham group, 6.15 ± 0.119 in the Taurine group, 5.02 ± 0.62 in the ischemia group, and 5.36 ± 0.384 in the treatment group. Less intracellular edema and inflammatory cell reaction were observed in histologic examination in favor of treatment group. (p < 0.01)ConclusionTaurine decreased myocardial damage during cold ischemic period following global myocardial ischemia.
Highlights
The aim of the present study was to investigate the cardioprotective effect of Taurine on the donor hearts during cold ischemic period
When average values were examined, the lowest value of MDA level was recorded in Taurine group and the highest value was recorded in the ischemia group
Superoxide dismutase (SOD) values that decreased in the sham Group were increased in the Treatment group to which Taurine was administered, and this difference between the ischemia group and the treatment group was found to be statistically significant (p < 0.001)
Summary
The aim of the present study was to investigate the cardioprotective effect of Taurine on the donor hearts during cold ischemic period. Hypothermic, cardioplegic arrest followed by cold transport is a common procedure for preservation of explanted hearts. This procedure is the main practical method used for preserving donor organs in many transplant centers [1]. There is no perfect protection method for donor organs currently. Organ functions can be improved by minimizing the myocardial function during ischemia. For this purpose many studies have been performed to prolong this ischemic time or protect the organs in this deleterious process
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
