Abstract
BackgroundHuman cytomegalovirus (HCMV) has been associated with malignant gliomas. The purpose of the present study was to investigate the presence of HCMV in common non-glial tumors of the central nervous system (CNS) and to determine whether it is a glioma-specific phenomenon.MethodsUsing HCMV-specific immunohistochemical staining, HCMV proteins IE1–72 and pp65 were examined in 65 meningiomas (benign, atypical and malignant), 45 pituitary adenomas, 20 cavernous hemangiomas, and 30 metastatic carcinomas specimens. HCMV DNA was also measured in these tumor tissues and the peripheral blood from patients using nested PCR.ResultsIn meningioma, IE1–72 was detected in 3.1% (2/65) and pp65 was detected in 4.6% (3/65), whereas no IE1–72 and pp65 were detected in atypical and malignant meningioma. A low level of IE1–72 immunoreactivity 6.7% (2/30) was detected in metastatic carcinoma; pp65 was not detected. No HCMV components were detected in pituitary adenoma and cavernous hemangioma. The results of immunohistochemical staining were confirmed by HCMV-specific PCR. HCMV DNA was not detected in the peripheral blood of the non-glial CNS tumors patients.ConclusionsOur results demonstrate that the presence of HCMV components is not an entirely glioma-specific phenomenon, and that HCMV is present in a low percentage in some non-glioma CNS tumors. Comparing HCMV-positive non-glial CNS tumors with HCMV-positive gliomas may cast light on the mechanism and role of HCMV in CNS tumors.
Highlights
Human cytomegalovirus (HCMV) has been associated with malignant gliomas
If a similar microenvironment exits in non-glial tumors of the central nervous system (CNS), it is possible that HCMV components might be present in these tumors, and local HCMV infection may not be a glioma-specific phenomenon
Immunohistochemical detection of HCMV IE1–72 proteins Immunohistochemical staining was used to detect whether HCMV proteins are expressed in a series of common nonglial CNS tumors including meningioma, pituitary adenoma, cavernous hemangioma, and metastatic carcinoma
Summary
The purpose of the present study was to investigate the presence of HCMV in common non-glial tumors of the central nervous system (CNS) and to determine whether it is a glioma-specific phenomenon. Our previous study detected HCMV IE1 immunoreactivity in 76.1% of glioma specimens of various grades, and pp in 65.7%, whereas. Our previous study suggested that the presence and role of HCMV in gliomas may be dependent upon the specific local tumor microenvironment [11]. If a similar microenvironment exits in non-glial tumors of the CNS, it is possible that HCMV components might be present in these tumors, and local HCMV infection may not be a glioma-specific phenomenon. The purpose of this study was to investigate the presence of HCMV components in common non-glial CNS tumors
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
