Abstract
Changes in the gibberellin A 4 (GA 4) content during culture of isolated apple embryos are dependent on the stage of seed after-ripening. Similarly, the intensity of incorporation of labelled gibberellins precursor into the GA 4 fraction depends to a high degree on the physiological state of seeds and correlates with the content of this hormone. The increase of endogenous GA 4 level was observed during the culture of embryos isolated from seeds stratified shorter than 40 days. The intensive incorporation of [ 14C]-mevalonate into a fraction co-chromatographed with GA 4 was stated during culture of such embryos. This incorporation was inhibited by 2-isopropyl-4-dimethylamino-5-methylphenyl-piperidinecarboxylate methyl chloride (AMO-1618), an inhibitor of gibberellins biosynthesis. The same treatment inhibits also the germination of embryos from seeds stratified for the same period. The suggestion was drawn concerning the role of the GA 4 biosynthesis in the removal of embryonal dormancy of apple seeds.
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