Is the dendritic organ of the striped eel catfish Plotosus lineatus an ammonia excretory organ?

Abstract

The dendritic organ (DO) is a salt secretory organ in the Plotosidae marine catfishes. The potential role of the DO in ammonia excretion was investigated by examining the effects of salinity [brackishwater (BW 3‰), seawater (SW 34‰) and hypersaline water (HSW 60‰)] acclimation and DO ligation on ammonia excretion and ammonia transporter expression by immunohistochemistry (IHC), immunoblotting (IB) and qPCR. Ammonia flux rates (JAmm) were significantly lower in BW compared to SW and HSW. DO ligation resulted in a significantly lower JAmm in SW but not BW fish. IHC demonstrated apical and basolateral localization of Rhesus-associated glycoprotein (Rhag-like) and Rhbg-like proteins, respectively, in parenchymal cells of the DO acini. In the gills, which are the primary site of ammonia excretion in teleost fishes, IHC showed an apical localization of Rhag-like protein in some Na+/K+-ATPase (NKA) immunoreactive (IR) cells limited to a few interlamellar regions of the filament and, in both apical and basolateral membranes of pillar cells irrespective of treatment group. In gills, the distribution of NKA-IR cells showed no salinity and/or ligation dependency. IB of Rhag and Rhbg-like proteins was found only in the gills and expression levels did not change with salinity but ligation in BW decreased Rhbg-like levels. Although Rhcg was not detected with heterologous antibodies, rhcg1 mRNA expression was detected in both gills and DO. HSW was associated with the lowest expression in DO and ligations in SW and BW were without effect on branchial expression levels. Taken together these results indicate the DO potentially has a physiological role in ammonia excretion under SW conditions.