Abstract

Environmental context The greenhouse gas nitrous oxide is produced by bacteria and emitted from terrestrial and aquatic environments; the origin of this compound can be determined by its 15N intramolecular distribution (site preference). The site preference of nitrous oxide was characterised experimentally in bacterial denitrifying communities under controlled conditions. This study shows the importance of the last step of denitrification on the site preference values, and that complementary methods are necessary to identify the sources of nitrous oxide. Abstract Site preference values of nitrous oxide emitted during different steps of benthic denitrification were determined. Compared to that of nitrous oxide as end product, the site preference during complete denitrification presents a large variation, due to the final step, and is highly correlated with nitrate reduction rate. The nitrous oxide reduction step appears decisive on the site preference values.

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