Is Rhodnius prolixus (Triatominae) invading houses in central Brazil?

Abstract

Sylvatic triatomines of the genus Rhodnius commonly fly into houses in Latin America, maintaining the risk of Chagas disease transmission in spite of control efforts. In the recent past, adult bugs collected inside houses in central Brazil were identified as R. prolixus, a primary disease vector whose natural geographical range excludes this region. Three nearly sibling species ( R. neglectus, R. nasutus, and R. robustus), secondary vectors with limited epidemiological significance, occur naturally south of the Brazilian Amazon. The specific status of Rhodnius specimens found inside houses in central Brazil is therefore an epidemiologically important (and still debated) issue. We used wing and head geometric morphometrics to investigate the taxonomic status of 230 adult specimens representing all four ‘ R. prolixus group’ species (19 populations from palm trees, domiciles, and reference laboratory colonies). Discriminant analyses of shape variation allowed for an almost perfect reclassification of individuals to their putative species. Shape patterning revealed no consistent differences between most specimens collected inside houses in central Brazil and R. neglectus, and showed that R. robustus and R. neglectus occur sympatrically (and fly into houses) in southern Amazonia. Furthermore, all Brazilian specimens clearly differed from our reference R. prolixus population. Using geometric morphometrics, we confidently ascribed individual triatomines to their species within the problematic ‘ R. prolixus group’, illustrating the potential value of this approach in entomological surveillance. Our results strongly support the idea that R. neglectus, and not R. prolixus, is the species invading houses in central Brazil.