Abstract
Purpose: The unusual ratio of tear electrolytes, as compared to serum values, and the known significance of tear osmolarity has driven interest in tear electrolyte composition. Although laboratory analytical techniques for electrolytes are available, sample size and sensitivity inhibit their use in routine tear analysis. In consequence comparative studies of tear electrolyte concentrations (as distinct from gross osmolarity measurements) are rarely carried out in healthy and compromised eyes. This study investigates the applicability of ion chromatography to analysis of tear electrolytes on single (<5 μl) tear samples and compares values obtained using microcapillary, Schirmer strip and tear envelope sampling.
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