Abstract
BackgroundFor a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough.MethodsTo confirm whether a positive CAMP test is a requirement for the identification of S.agalactiae, five suspected CAMP-negative S.agalactiae isolates from two hospitals, confirmed as Gram-positive and catalase-negative streptococci, were verified by the CAMP test in three batches of plates from two manufacturers and identified by the Phoenix system, MALDI-TOF MS, the PCR assay and the 16S rDNA gene sequencing.ResultsAll five suspected strains were S.agalactiae, four of which were CAMP-negative and one of which was not S.agalactiae by the PCR assay.ConclusionsA positive CAMP test was lacking sensitivity for the identification of S.agalactiae, and the question of whether the cfb gene is worthy of targeting should be further studied.
Highlights
For a long time, the Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough
The CAMP (Christie, Atkinson, Munch, Peterson) test is used in some laboratories to verify whether bacteria have enhanced staphylococcus beta-lysis activity test, which has long been considered as a key, confirmed test for the identification of Group B Streptococcus (GBS) [9,10,11,12]
Strain No.51 could not be identified as GBS by the PCR assay only, all other four suspected strains were GBS by all four methods
Summary
The Christie-Atkinson-Munch-Peterson (CAMP) test has been a standard test for the identification of Streptococcus agalactiae, and a positive result for S.agalactiae has been considered sensitive enough. Conclusions: A positive CAMP test was lacking sensitivity for the identification of S.agalactiae, and the question of whether the cfb gene is worthy of targeting should be further studied. The CAMP (Christie, Atkinson, Munch, Peterson) test is used in some laboratories to verify whether bacteria have enhanced staphylococcus beta-lysis activity test, which has long been considered as a key, confirmed test for the identification of GBS [9,10,11,12]. Some PCR-based assays for GBS have targeted the cfb gene, which encodes the CAMP factor [13, 14].
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