Abstract
The relative integrative recombination frequencies of the F plasmid IS 2 and IS 3 elements were determined at two Escherichia coli K-12 chromosomal sites by hybridization analysis of Hfr DNA. The sequence organizations of the independent Hfr strains formed by F integration atα 3β 3 indicate that the relative recombinational frequencies at the two F plasmid IS 3 elementsα 1β 1 and α 2β 2 are not significantly different. A comparison of the relative recombinational frequencies of the IS 2 and IS 3 elements of F was provided by analysis of DNA from Hfr strains having F integrated betweenlacandproC (i.e., at the IS 2 or at the IS 3 element (α 5β 5) located in this region). No instances of F integration atα 5β 5 were detected, indicating that integrative recombination at IS 2 is significantly more frequent than at IS 3 in this chromosomal region.
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