Irradiation with 365 nm and 405 nm wavelength shows differences in DNA damage of swine pancreatic islets.

M Klak,P Kowalska,J Idaszek,M Gomółka,A Dobrzyń,G Tymicki,M Wszoła,W Święszkowski,P Cywoniuk,T Bryniarski,A Berman,K Kosowska,T Dobrzański,Vahid Serpooshan

doi:10.1371/journal.pone.0235052

Abstract

Introduction3D printing is being used more extensively in modern biomedicine. One of the problems is selecting a proper crosslinking method of bioprinted material. Amongst currently used techniques we can distinguish: physical crosslinking (e.g. Ca2+ and Sr2+) and chemical crosslinking–the UV light crosslinking causing the biggest discussion. UV radiation is selectively absorbed by DNA, mainly in the UV-B region but also (to some extent) in UV-A and UV-C regions. DNA excitement results in typical photoproducts. The amount of strand breaks may vary depending on the period of exposition, it can also differ when cells undergo incubation after radiation.AimThe aim of this study was to show whether and how the time of irradiation with 405 nm and 365 nm wavelengths affect DNA damage in cell lines and micro-organs (pancreatic islets).Materials and methodsThe degree of DNA damage caused by different wavelengths of radiation (405 nm and 365 nm) was evaluated by a comet assay. The test was performed on fibroblasts, alpha cells, beta cells and porcine pancreatic islets after 24 hours incubation period. Samples without radiation treatment were selected as a control group. Results analysis consisted of determining the percent of cells with damaged DNA and the tail intensity evaluation.ResultsThe degree of DNA damage in pancreatic islets after exposure to 405 nm wavelength oscillated between 2% and 6% depending on the tested time period (10 – 300 seconds). However, treating islets using 365 nm wavelength resulted in damage up to 50%. This clearly shows significantly less damage when using 405 nm wavelength. Similar results were obtained for the tested cell lines.ConclusionsCrosslinking with 405 nm is better for pancreatic islets than crosslinking with 365 nm UV light.

Highlights

AimThe aim of this study was to show whether and how the time of irradiation with 405 nm and 365 nm wavelengths affect DNA damage in cell lines and micro-organs
The degree of DNA damage in pancreatic islets after exposure to 405 nm wavelength oscillated between 2% and 6% depending on the tested time period (10 – 300 seconds)
Treating pancreatic islet cells with 405 nm wavelength for 120 seconds resulted in 3.5% cells with damaged DNA while 300 seconds of 405 nm radiation caused DNA damage in 5.5% of cells

Summary

Introduction

AimThe aim of this study was to show whether and how the time of irradiation with 405 nm and 365 nm wavelengths affect DNA damage in cell lines and micro-organs (pancreatic islets). One of the most important steps in the bioprinting procedure is choosing the right crosslinking method of the bioprinted material The reason for this step is maintaining the correct physical form and good shape of bioprinted construct that will allow its implementation in the future [4]. It can be achieved using a few different techniques. UV light can be a good crosslinking agent while printing without cells but it can be harmful when a decision to implement cell-laden bioink is made This is because UV light is considered to be a DNA damaging agent [6,7]

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