Abstract
Increasing the immunogenicity of tumors is considered to be an effective means to improve the synergistic immune effect of radiotherapy. Carbon ions have become ideal radiation for combined immunotherapy due to their particular radiobiological advantages. However, the difference in time and dose of immunogenic changes induced by Carbon ions and X-rays has not yet been fully clarified. To further explore the immunogenicity differences between carbon ions and X-rays induced by radiation in different “time windows” and “dose windows.” In this study, we used principal component analysis (PCA) to screen out the marker genes from the single-cell RNA-sequencing (scRNA-seq) of CD8+ T cells and constructed a protein-protein interaction (PPI) network. Also, ELISA was used to test the exposure levels of HMGB1, IL-10, and TGF-β under different “time windows” and “dose windows” of irradiation with X-rays and carbon ions for A549, H520, and Lewis Lung Carcinoma (LLC) cell lines. The results demonstrated that different marker genes were involved in different processes of immune effect. HMGB1 was significantly enriched in the activated state, while the immunosuppressive factors TGF-β and IL-10 were mainly enriched in the non-functional state. Both X-rays and Carbon ions promoted the exposure of HMGB1, IL-10, and TGF-β in a time-dependent manner. X-rays but not Carbon ions increased the HMGB1 exposure level in a dose-dependent manner. Besides, compared with X-rays, carbon ions increased the exposure of HMGB1 while relatively reduced the exposure levels of immunosuppressive factors IL-10 and TGF-β. Therefore, we speculate that Carbon ions may be more advantageous than conventional X-rays in inducing immune effects.
Highlights
Radiotherapy (RT) is the primary treatment for lung cancer, which first-line treatment accounts for ∼30% of all newly diagnosed patients [1]
Construction of protein-protein interaction (PPI) Network Based on Significantly differential expression genes (DEGs) in principal component analysis (PCA)
Based on the CD8+T cell scRNA-seq in lung cancer, we used PCA to screen out the DEGs expressed in the resting state (PC_0), activated state (PC_1), and non-functional state (PC_2) clusters
Summary
Radiotherapy (RT) is the primary treatment for lung cancer, which first-line treatment accounts for ∼30% of all newly diagnosed patients [1]. Immunotherapy has become the most promising and effective treatment for Radiation-Induced Immunogenicity Changes lung cancer [3]. In addition to activating immunity, radiation has an immunosuppressive effect [6], including the recruitment or polarization of immunosuppressive cytokines, immune checkpoint molecules, and suppressive immune cell subtypes [7]. Carbon ions have significant radiobiological advantages over conventional X-rays [8, 9], and the direct killing effect on radiation-resistant tumor cells is stronger than conventional X-rays 2-3 times [5, 10]. It is essential to analyze the immunogenic changes induced by two kinds of radiation in tumor cells to improve radioimmunity
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
