IRoot SP Promotes Osteo/Odontogenesis of Bone Marrow Mesenchymal Stem Cells via Activation of NF-κB and MAPK Signaling Pathways.

Xiao Wu,Jiamin Lu,Ming Yan,Minxia Bian,Xingyun Ge,Lin Fu,Yuzhi Li,Jinhua Yu,Francisco J Rodr Guez Lozano

doi:10.1155/2020/6673467

Abstract

The regeneration of bone and tooth tissues, and related cellular therapies, has attracted widespread attention. Bone marrow mesenchymal stem cells (BMSCs) are potential candidates for such regeneration. iRoot SP is a premixed bioceramic root canal sealer widely used in clinical settings. However, the effect of iRoot SP on the biological features of BMSCs has not been elucidated. In the present study, we found that 0.2 mg/ml iRoot SP conditioned medium promoted osteo/odontogenic differentiation and enhanced mineralization of BMSCs without affecting the proliferative ability. Mechanistically, the NF-κB and MAPK signaling pathways were activated in SP-treated BMSCs, and differentiation was inhibited when cultured with the specific inhibitor. Taken together, these findings demonstrate that iRoot SP promotes osteo/odontogenic differentiation of BMSCs via the NF-κB and MAPK signaling pathways, which could provide a new theoretical basis for clinical applications of iRoot SP and a new therapeutic target for the regeneration of bone and tooth tissue in the future.

Highlights

The pulp tissue of teeth can be affected by trauma, dental caries, and anatomic variation, leading to clinical cases of pulpitis and periapical periodontitis [1]
The results revealed that iRoot SP promotes the osteo/odontogenic capacity of Bone marrow mesenchymal stem cells (BMSCs) via the NF-κB and mitogen-activated protein kinase (MAPK) signaling cascades
BMSCs treated with 0.2 mg/ml SPshowed the highest upregulation of Alkaline Phosphatase (ALP) enzyme activity in comparison with the other groups (p < :001; Figure 1(c))

Summary

Introduction

The pulp tissue of teeth can be affected by trauma, dental caries, and anatomic variation, leading to clinical cases of pulpitis and periapical periodontitis [1]. Various materials, including zinc oxide eugenol, resin, glass ionomer, and calcium hydroxide, have been utilized as root canal sealers [3]. When a sealer like iRoot SP is used, it is placed in the canal and confined there It may be extruded through the apical foramen, or it may release chemicals into the periapical tissue [9]. Apical surgery is commonly performed for refractory chronic apical periodontitis During this procedure, it is inevitable that sealers will come into contact with the surrounding tissues and cells. It is inevitable that sealers will come into contact with the surrounding tissues and cells Due to their superior proliferative and multidirectional differentiation abilities, bone marrow mesenchymal stem cells (BMSCs) play a major role in stem. The results revealed that iRoot SP promotes the osteo/odontogenic capacity of BMSCs via the NF-κB and MAPK signaling cascades

Materials and Methods

Results

Discussion

Conflicts of Interest