Abstract
BackgroundiRoot BP Plus is a novel bioceramic endodontic material. Recently, it has been considered as an alternative to MTA which is the most popular scaffold cover during regenerative endodontic therapy. This study aimed to evaluate the effects of iRoot BP Plus on the osteo/odontogenic capacity of bone marrow mesenchymal stem cells (BMMSCs), including the underlying mechanisms.MethodsBMMSCs were collected by a whole marrow method and treated with iRoot BP Plus-conditioned medium (BP-CM). The proliferation ability was evaluated by cell counting kit 8 and flow cytometry. Complete medium was used as a blank control, and 2 mg/ml MTA-conditioned medium was served as a positive control. Alkaline phosphatase (ALP) activity assay, ALP staining, western blot, real-time RT-PCR, Alizarin Red S staining, and immunofluorescence staining were performed to explore the osteo/odontogenic potential and the involvement of MAPK pathways. Besides, autophagy was investigated by western blot, immunofluorescence staining, and transmission electron microscopy.Results
Highlights
Dental pulp exposure caused by carious or trauma in immature teeth always leads to necrosis and apical periodontitis
Conclusion iRoot BP Plus promotes the osteo/odontogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) via the MAPK pathways and autophagy, which are important for its application in bone and tooth tissue regeneration engineering
Our findings revealed that iRoot BP Plus could enhance the osteogenic and odontogenic potential of BMMSCs through the MAPK pathways and autophagy
Summary
Dental pulp exposure caused by carious or trauma in immature teeth always leads to necrosis and apical periodontitis. The risk of root fracture will increase due to thin root walls and shortened root length resulting from arrested root development [1]. In these cases, regenerative endodontic therapy is the most desirable healing response because of the pulp-dentine complex regeneration and further root development after treatment. Mineral trioxide aggregate (MTA), a calcium silicate-based biomaterial, is used extensively for scaffold cover during regenerative endodontic therapy. IRoot BP Plus is a novel bioceramic endodontic material. It has been considered as an alternative to MTA which is the most popular scaffold cover during regenerative endodontic therapy. This study aimed to evaluate the effects of iRoot BP Plus on the osteo/odontogenic capacity of bone marrow mesenchymal stem cells (BMMSCs), including the underlying mechanisms
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
