Abstract
To investigate the effects of dietary iron (Fe) on manganese (Mn) metabolism, 24 weaned pigs (21 d old) were blocked by litter and weight and randomly assigned to the following treatments: 1) no supplemental Fe [low Fe (L-Fe)]; 2) 100 mg supplemental Fe/kg [adequate Fe (A-Fe)]; and 3) 500 mg supplemental Fe/kg [high Fe (H-Fe)]. The basal diet was analyzed to contain 20 mg Fe/kg. Tissues were harvested after 32 d of feeding. Daily gain (least square means ± SEM) was greater in A-Fe pigs (328.3 ± 29.9 g/d) than in L-Fe pigs (224.0 ± 11.2 g/d). Hemoglobin concentrations on d 32 were lower in L-Fe pigs (62 ± 3.5 g/L) than in A-Fe pigs (128 ± 5.6 g/L) and did not differ between pigs fed A-Fe and H-Fe (133 ± 12.0 g/L). Liver Fe increased with increasing dietary Fe. Relative hepatic hepcidin expression was greater in pigs fed A-Fe and H-Fe than in those fed L-Fe. Relative expressions of duodenal divalent metal transporter 1 (DMT1) and solute carrier family 39 member 14 (ZIP14) were increased in L-Fe pigs compared with H-Fe pigs. Liver copper (Cu) was higher in L-Fe (0.56 ± 0.04 mmol/kg) and H-Fe (0.58 ± 0.04 mmol/kg) pigs than in A-Fe pigs (0.40 ± 0.04 mmol/kg). Liver Mn was lower in H-Fe pigs (0.15 ± 0.01 mmol/kg) than in A-Fe (0.23 ± 0.02 mmol/kg) or L-Fe pigs (0.20 ± 0.02 mmol/kg). Duodenal Mn concentrations were greater in L-Fe pigs than in A-Fe or H-Fe pigs. Fe deficiency in pigs increased gene expression of duodenal metal transporters (DMT1 and ZIP14) and supplementation with H-Fe reduced expression of DMT1 and ZIP14, which may have decreased absorption of Mn.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.