Abstract
In vitro uptake of radioiron by suspensions of isolated rat duodenal mucosal cells has been examined. The cells bind iron avidly, with uptakes of 15 to 20% after 20 min of incubation. Uptake is dependent upon temperature, pH, and iron and cell concentration. It is unaffected by inhibitors of cellular respiration but is blocked in a concentration-dependent fashion by iron-chelating agents. Iron-deficient cells take up iron at the same rate as normal cells. Binding by brush borders appears to account for 50 to 60% of the total uptake by isolated cells. In vitro radioiron uptake by isolated cells occurs by passive diffusion, is not regulated by the animal's iron status, and is primarily a function of bioavailability of iron in the incubating medium. If the model reflects the behavior of cells in vivo, the initial uptake of iron by the mucosa is a passive process which may serve to concentrate dilute luminal iron at the mucosal surface in preparation for absorption.
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