Abstract
When ferritin solutions containing 8–1257Fe atoms/molecule are mixed after iron loading, with ferritin containing a small56Fe core (56Fe-ferritins) or with an equal volume of NaCl solution (controls) and the mixtures are frozen in liquid nitrogen (LN) at specified times, the spectra of the56Fe-ferritins show that almost all57Fe is attached to large clusters, whereas in the controls all57Fe is present as isolated atoma,57Fe dimers or small clusters and no large57Fe containing clusters are observed. This shows that57Fe present as solitary or dimer species in ferritin migrates to ferritin molecules containing larger56Fe clusters.
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