Iron stress restricts photosynthetic intersystem electron transport in Synechococcus sp. PCC 7942

Abstract

Although exposure of Synechococcus sp. PCC 7942 to iron stress induced the accumulation of the isiA gene product (CP43′) compared with non-stressed controls, immunodetection of the N-terminus of cytochrome (Cyt) f indicated that iron stress not only reduced the content of the 40 kDa, heme-binding, Cyt f polypeptide by 32% but it also specifically induced the accumulation of a new, 23 kDa, non-heme-binding, putative Cyt f polypeptide. Concomitantly, iron stress restricted intersystem electron transport based on the in vivo reduction of P700 +, monitored as Δ A 820/ A 820 in the presence and absence of electron transport inhibitors, as well as the inhibition of the Emerson enhancement effect on O 2 evolution. However, iron stress appeared to be associated with enhanced rates of PS I cyclic electron transport, low rates of PS I-driven photoreduction of NADP + but comparable rates for PS II+PS I photoreduction of NADP + relative to controls. We hypothesize that Synechococcus sp. PCC 7942 exhibits a dynamic capacity to uncouple PS II and PS I electron transport, which may allow for the higher than expected growth rates observed during iron stress.