Iron-rich Candida utilis improves intestinal health in weanling piglets.

Abstract

This study aimed to investigate the effects of substituting inorganic iron in the diet of weanling piglets with iron-rich Candida utilis on gut morphology, immunity, barrier, and microbiota. Seventy-two healthy 28-day-old Duroc×Landrace×Yorkshire desexed male weanling piglets were randomly assigned to 2 groups (n=6), with 6 pens per group and 6 piglets in each pen. The control group was fed a basal diet containing ferrous sulfate (104mg kg-1 iron), while the experimental group was fed a basal diet supplemented with iron-rich C. utilis (104mg kg-1 iron). The results show that the growth performance of weanling piglets showed no significantly differences (P>0.05). Iron-rich C. utilis significantly elevated villus height and decreased crypt depth in the duodenum and jejunum (P<0.05). Additionally, there was a significant increase in SIgA content, a down-regulated of pro-inflammatory factors expression, and an up-regulated of anti-inflammatory factors expression in the jejunum and ileum of piglets fed iron-rich C. utilis (P<0.05). The mRNA expression levels of ZO-1, Claudin-1, Occludin, and Mucin2 in the jejunum were significantly increased by iron-rich C. utilis, and were significantly increased ZO-1 and Claudin-1 in the ileum (P<0.05). The colonic microbiota, however, was not significantly affected by iron-rich C. utilis (P>0.05). Iron-rich C. utilis improved intestinal morphology and structure, as well as intestinal immunity and intestinal barrier function.