Abstract
The Saccharomyces cerevisiae iron-responsive transcription factor, Aft1, has a well established role in regulating iron homeostasis through the transcriptional induction of iron-regulon genes. However, recent studies have implicated Aft1 in other cellular processes independent of iron regulation such as chromosome stability. In addition, chromosome spreads and two-hybrid data suggest that Aft1 interacts with and co-localizes with kinetochore proteins; however, the cellular implications of this have not been established. Here, we demonstrate that Aft1 associates with the kinetochore complex through Iml3. Furthermore, like Iml3, Aft1 is required for the increased association of cohesin with pericentric chromatin, which is required to resist microtubule tension, and aft1Δ cells display chromosome segregation defects in meiosis. Our work defines a new role for Aft1 in chromosome stability and transmission.
Highlights
The budding yeast transcription factor Aft1 has been implicated in chromosome stability
Aft1 Interacts with the Kinetochore Protein Iml3—To elucidate the function of Aft1 in chromosome stability, we first sought to identify the individual kinetochore protein or protein subcomplex that interacts with Aft1
In an iml3⌬ background that maintains the Chl4-Ctf19 interaction, albeit in a reduced fashion [15, 19], the Aft1-Ctf19 interaction was disrupted (Fig. 1B, lane 6). These results suggest that Aft1 is co-purifying Chl4 and Ctf19 through Iml3
Summary
The budding yeast transcription factor Aft has been implicated in chromosome stability. The paralog of AFT1, AFT2, and other iron-regulon genes were not identified in the genome-wide studies that implicated Aft in chromosome stability [4, 6, 7] nor are aft2⌬ mutant cells sensitive to benomyl treatment [4]. Overall, this suggests that the role of Aft in chromosome stability is not related to regulation of the iron-regulon or iron homeostasis. We discover that Aft interacts with Iml and has a role in promoting pericentric cohesin
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