Iron oxide nanoparticles alleviate arsenic phytotoxicity in rice by improving iron uptake, oxidative stress tolerance and diminishing arsenic accumulation

Abstract

The food chain contaminated with arsenic (As) has developed a hazardous threat to the growth and development of plants, animals and humans. The present study was conducted to examine the application of iron oxide nanoparticles (FeNPs) on biochemical and molecular traits of roots and leaves of rice plants under As phytotoxicity. The results showed that As reduced the accumulation of Fe in roots and leaves and thus reduced photosynthetic pigments and growth of rice plants. As stress enhanced the accumulation of hydrogen peroxide, superoxide anion and methylglyoxal by increasing the accumulation of As in roots and leaves, resulting in damage to membrane lipids and raised electrolyte leakage (EL). However, FeNPs strengthen the glyoxalase system and antioxidant enzymes, thereby alleviating oxidative stress and reducing EL. FeNPs protected plant cells from As phytotoxicity by enhancing the accumulation of chelating agents (proline, glutathione and phytochelatins) and the sequestration and immobilization of As in the vacuoles and the cell walls. FeNPs downregulated the expression of genes involved in As uptake and translocation (Lsi1 and Lsi2) and, consequently, reduced As accumulation in the roots and leaves of As-stressed plants. FeNPs also improved the accumulation of Fe in the roots and leaves by modulating the expression of genes that regulate Fe uptake and its transport to leaves (IRT1, IRT2, YSL2, YSL13, FRDL1, DMAS1, NAS2 and NAS3), resulting in the restoration of photosynthetic pigments and the growth of As-stressed plants. Our findings authenticate the role of FeNPs in diminishing As phytotoxicity on rice.