Iron Oxide/Chitosan Magnetic Nanocomposite Immobilized Manganese Peroxidase for Decolorization of Textile Wastewater

Saifeldin M Siddeeg,Faouzi Ben Rebah,Wissem Mnif,Mohamed A Tahoon

doi:10.3390/pr8010005

Abstract

Because of its effectiveness in organic pollutant degradation, manganese peroxidase (MnP) enzyme has attracted significant attention in recent years regarding its use for wastewater treatment. Herein, MnP was extracted from Anthracophyllum discolor fungi and immobilized on the surface of magnetic nanocomposite Fe3O4/chitosan. The prepared nanocomposite offered a high surface area for MnP immobilization. The influence of several environmental factors like temperature, pH, as well as storage duration on the activity of the extracted enzyme has been studied. Fourier transmission infrared spectroscopy (FT-IR), scanning electron microscope (SEM), X-ray diffraction (XRD), and transmission electron microscope (TEM) techniques were used for the characterization of the prepared MnP/Fe3O4/chitosan nanocomposite. The efficiencies of the prepared MnP/Fe3O4/chitosan nanocomposite for the elimination of reactive orange 16 (RO 16) and methylene blue (MB) industrial dyes were determined. According to the results, the immobilization of MnP on Fe3O4/chitosan nanocomposite increases its capacity to decolorize MB and RO 16. This nanocomposite allowed the removal of 96% ± 2% and 98% ± 2% of MB and RO 16, respectively. The reusability of the synthesized nanocomposite was studied for five successive cycles showing the ability to retain its efficiency even after five cycles. Thus, the prepared MnP/Fe3O4/chitosan nanocomposite has potential to be a promising material for textile wastewater bioremediation.

Highlights

Urbanization and contamination of surface drinking water resources have led to a crucial problem concerning the provision of safe water supplies in our contemporary world [1]
In the present study we examined the efficiency of manganese peroxidase (MnP) enzyme extracted from the white root fungus of Anthracophyllum discolor toward the elimination of organic dyes reactive orange 16 (RO 16) and methylene blue (MB) from textile wastewater
scanning electron microscope (SEM) showed a homogeneous distribution of Fe3 O4 nanoparticles, which is related to the presence of chitosan offering high surface for the immobilization of the MnP enzyme

Summary

Introduction

Urbanization and contamination of surface drinking water resources have led to a crucial problem concerning the provision of safe water supplies in our contemporary world [1]. The industrial release of MB can disrupt ecosystem balance and damage the environment, due to its carcinogenic effects [3,4]. These effects are accompanied by several symptoms including severe headaches, skin irritation, and acute diarrhea [5]. Azo dyes have been shown to have mutagenic and carcinogenic effects [7,8]. Dyes must be removed before effluent discharge into the environment, as these molecules are hard to decompose in natural conditions (pH, temperature, etc.) or with conventional methods of treatment [9]

Methods

Results

Conclusion