Abstract
The effects of ammonium (NH 4 + -N) and nitrate (NO 3 − -N) were examined on Fe fractions and FeCN (ferricyanide) reductase activity in intact root tips (0–3 cm) of young maize (Zea mays L. cv. Lenz) in solution culture by using short-term experiment under controlled Fe deficiency conditions (containing high HCO 3 − concentration in preculture solution). The results showed that Fe(II) concentrations in root tip apoplast of maize were only 20–40 nmol/g FW which accounted for 7%–13% of total Fe. Most of Fe in root tips existed as Fe(III) compounds. Imposition of the roots to NH 4 ±N or NO 3 −N for 60 min led to an increase of Fe(II) in root tip apoplast. NH 4 ±N led to an increased concentration of Fe(II) and exchangeable Fe (Fe(II) and Fe (III)) in root tips, while NO 3 − -N increased FeCN reductase activity. The relationship between pH and Fe fractions, FeCN reductase activity was also discussed.
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