Abstract
The facile detection of total antioxidant capacity (TAC) is limited by in-situ analysis, because it usually requires complex laboratory equipments. Here, a colorimetric assay for TAC detection is developed based on the peroxidase-like activity of iron-doped carbon nitride (Fe/NC) and the smartphone platform. The peroxidase-like activity of carbon nitride is greatly improved by the introduction of Fe atoms, and the active sites turn to Fe-Nx coordination groups in the Fe/NC. The inhibition mechanism of the chromogenic reaction for different kinds of antioxidants is also studied. The colorimetric assay is fabricated by the relationship of absorbance-color-antioxidant content and applied successfully to the TAC detection of several fruit juicesand commercial beverages. This work not only provides a promising approach for convenient in-situ TAC assay without the use of large instruments, but also expands the application of nanozymes in nutritional value assessment of foods.
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