Abstract
Adventitious root formation in stem cuttings is affected by exogenous and endogenous factors. The study assessed the effect of Fe(III)-EDDHA (ethylenediamine-N,N-bis 2-hydroxyphenyl acetic acid) on the rooting of 4 indol-3-butyric acid (IBA)-treated hardwood cuttings of the aromatic and medicinal species Rosmarinus officinalis. Cuttings treated with 0, 1000, 2000 or 3000 mg L−1 IBA were placed in pots filled with sand:perlite mixture and irrigated daily with nutrient solution pH 5.8, containing 0, 5, 10 or 20 µM Fe(III)-EDDHA. Ten days later, the number of new emerging roots were recorded. After 20 days, leaf photosynthetic pigments and morphological traits, including root number, fresh (FW) and dry weight (DW), shoot FW and DW, mean length of the longest roots, number of new shoots and new growth in old shoots, were measured. Finally, plants were transplanted to pots filled with a sand:soil mixture and survival was measured after 10 days. Results indicate that Fe application promotes root emergence and improves root and shoot biomass, leaf photosynthetic pigment concentrations and survival percentage. This indicates that using low concentrations of Fe(III)-EDDHA (5–20 µM) in the growth medium could be a good management strategy to facilitate the production of vigorous R. officinalis plants from hardwood cuttings.
Highlights
Salvatore La Bella and Adventitious root formation in plant cuttings is influenced by a large set of exogenous and endogenous factors [1]
Among the exogenous rooting factors, the auxin indol-3-butyric acid (IBA) is widely used to stimulate rooting processes in cuttings, because of its high ability to promote root initiation. This effect of IBA is thought to be due to its conversion in the plant tissue to indole-3-acetic acid (IAA), which is needed for the rooting process
Data shown here indicate that when Fe supplementation is not used, Results confirm that the application of IBA improves rooting in R. offici an IBA concentration of 1000 mg L− 1 appears to be adequate for R. officinalis, since higher
Summary
Salvatore La Bella and Adventitious root formation in plant cuttings is influenced by a large set of exogenous and endogenous factors [1]. Endogenous factors that could act as rooting co-factors and auxin transport modulators are transferred from the stock plants to the propagules [2]. Among the exogenous rooting factors, the auxin IBA (indole-3-butyric acid) is widely used to stimulate rooting processes in cuttings, because of its high ability to promote root initiation. This effect of IBA is thought to be due to its conversion in the plant tissue to indole-3-acetic acid (IAA), which is needed for the rooting process. Endogenous IAA can be readily oxidized in plants by peroxidase, but IBA is quite stable and is only slowly transported from the site of application at the base of the cuttings, resulting in a localized
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