Irisin Activates M1 Macrophage and Suppresses Th2-Type Immune Response in Rats with Pelvic Inflammatory Disease.

Zhenge Zhang,Chongyuan Zhang,Shuirong Zhang,Alamgeer Yuchi

doi:10.1155/2022/5215915

Abstract

Objective To investigate the mechanism of irisin to treat rats with acute pelvic inflammatory disease (APID). Methods Female rats were established as APID. Firstly, the content of IL-6, IL-8, TNF-α, and NF-kB was tested in rats' serum by enzyme linked immunosorbent assay (ELISA). Interferon-γ (IFN-γ) and IL-4 in the supernatant of pelvic homogenates were also detected. The mRNA expression of the inducible nitric oxide synthase (iNOS), TNF-α, chemokine ligand 1 (CXCL1), arginase-1(Arg1), and chitinase-3-like-3 (Chi313) genes in the pelvic cavity was detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). IFN-γ and IL-4 secreted by spleen CD4+T cells and CD8+T cells were counted by flow cytometry, and the ratio of IFN-γ/IL-4 in CD4+T cells and CD8+T cells in the spleen was also detected by flow cytometry. Results Irisin reduced the levels of IL-6, IL-8, TNF-α, and NF-kB in serum. Compared with the APID group, the expression level of IL-4 in the APID + Irisin group was reduced in the homogenate. At the same time, Irisin promotes the activation of M1 macrophages in the uterus, ovaries, and uterine tubes of rats with APID. Irisin also inhibited Th2-type immune response. Conclusions Irisin activates M1 macrophage and suppresses Th2-type immune response in APID rats.

Highlights

ObjectiveTo investigate the mechanism of irisin to treat rats with acute pelvic inflammatory disease (APID)
Acute pelvic inflammatory disease (APID) is one of the gynecological diseases with high incidence and great harm in clinic
APID is a type of gynecological disease that refers to pelvic cavity reproductive organs such as the uterus, uterine tubes, and pelvic inflammation [1, 2]. e main reason is the anaerobic bacteria infection

Summary

Objective

To investigate the mechanism of irisin to treat rats with acute pelvic inflammatory disease (APID). Interferon-c (IFN-c) and IL-4 in the supernatant of pelvic homogenates were detected. E mRNA expression of the inducible nitric oxide synthase (iNOS), TNF-α, chemokine ligand 1 (CXCL1), arginase-1(Arg1), and chitinase-3-like-3 (Chi313) genes in the pelvic cavity was detected by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Irisin reduced the levels of IL-6, IL-8, TNF-α, and NF-kB in serum. Compared with the APID group, the expression level of IL-4 in the APID + Irisin group was reduced in the homogenate. Irisin promotes the activation of M1 macrophages in the uterus, ovaries, and uterine tubes of rats with APID. Irisin activates M1 macrophage and suppresses 2-type immune response in APID rats

Introduction

Materials and Methods

Results