Abstract
Abstract A liquid chromatographic [HPLC] method for the determination of Albendazole (ABZ), Oxfendazole (OFZ), Oxibendazole (OXBZ), and Thiabendazole (TBZ) residues in milk at levels below 3 ppb has been developed. Samples were deproteinized with acetonitrile, defatted with hexane partition, and evaporated to constant volume. Following addition of acidified aqueous octane-1-sulfonate solution, the target benzimidazoles were selectively extracted as ion pairs into chloroform and analyzed on a reversed-phase C18, 5 μm, column. Overall recoveries were found to be 93.5 ± 2.6% for OFZ, 90.0 ± 1.3% for OXBZ, 85.7 ± 3% for ABZ, and 74.0 ± 2.6% for TBZ. Limited data on the depletion of OXBZ and ABZ in sheep milk were also generated.
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