Abstract
High-performance systems for ion-pair chromatography that permit the detection of down to 7 pmole (about 1 ng) of non-UV-absorbing samples have been constructed, the high detector response being obtained by using a stationary phase containing a counter ion of high absorbance. Amino acids, dipeptides and alkylamines are separated with naphthalene-2-sulphonate as counter ion. The systems have a high selectivity, and a change of the amino acid sequence if often sufficient for a complete chromatographic resolution of dipeptides. A comparison with batch extraction data showed that the retention of the cations, except the most hydrophobic, is due mainly to liquid-liquid distribution. The naphthalene-2-sulphonate systems have a very high stability under careful thermostatted conditions. Systems for the separation of anionic compounds with highly absorbing quaternary ammonium ions in the stationary phase are being investigated.
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