Ionization and fragmentation of monochloro‐isomers of 3‐hydroxy‐2‐phenyl‐4(1H)‐quinolinone

Abstract

Electron ionization (EI), methane chemical ionization (CI), and collision-induced dissociation (CID) mass spectra of complete series of positional monochloro-isomers of 3-hydroxy-2-phenyl-4(1H)-quinolinone are evaluated and discussed. It is shown that in the CI experiments, in addition to the protonated precursor molecules, odd-electron molecular ions are formed and this affects the appearance of the CID spectra. The influence of different direct probes and other experimental parameters such as the pressure of the reagent gas, isolation width, or collision energy was studied. EI, CI and CID spectra of the positional isomers show essentially the same fragmentation pathways but comparisons of the relative signal intensities of various product ions reveal some positional effects. Different isomers are also distinguished. The compounds can be divided into two groups using diagnostic ions (chloro substitution of the quinolinone moiety or the phenyl ring) or identified using a created spectral database. It was demonstrated that the reproducibility of the CID spectra is fully satisfactory for isomer identification, and that the created database can be applied for comparison of spectra measured over an extended time period (1 month) or spectra obtained during the direct analysis of a reaction mixture extract. Explanation of the fragmentation of the isomers is supported by exploratory density functional theory (DFT) calculations, e.g. rationalization of the relatively higher importance of the M+.-H.-Cl.-CO fragmentation pathway during EI than during CID, and vice versa for the pathway M+.-Cl.-CO.