Abstract
Kinetic studies of the reduction of plastocyanin from spinach chloroplasts by ascorbate were carried out using a stopped flow apparatus. The reduction of spinach plastocyanin by ascorbate followed first-order kinetics in the reductant with a rate constant k = 415 M-1.S-1 [20 degrees C, 0.1 M NaCl, pH 8.0 (10 mM Tris-HCl)]. The second-order rate constants for the reduction of plastocyanin by ascorbate increased dramatically with increasing pH and ionic strength. The cationic micelles of cetyltrimethylammonium bromide also accelerated this electron-transfer reaction. The importance of electrostatic interactions between plastocyanin and ascorbate is discussed in relation to the photosynthetic electron-transport reactions.
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