Abstract
1. 1. The perfused midgut of intermolt (stage C 4) Homarus americanus had a net water flux (mucosa to serosa) of 32.4 μl · cm −2 · hr −1 and generated a small serosa-positive potential difference of 0.26 mV when bathed in normal lobster Ringer. 2. 2. Both potential difference and net water flux required Na + but not Cl −, were abolished by cyanide (10 −3 M) in the serosal bath, and were insensitive to ouabain (10 −4 M) when simultaneously applied to both sides of the epithelium. 3. 3. The results are consistent with the hypothesis that net water transport is linked to active transport of Na + across the lobster epithelium.
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