Ionic liquid-based multi-stage sugaring-out extraction of lactic acid from simulated broth and actual lignocellulosic fermentation broth

Xu Zhou,Zhilong Xiu,Yi Tong,Hongjun Zhan,Yaqin Sun,Haijun Liu,Xiaoyan Wang,Yi Li,Yang Xu

doi:10.1186/s40643-021-00481-4

Xu Zhou, Zhilong Xiu + Show 7 more

Open Access

https://doi.org/10.1186/s40643-021-00481-4

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Abstract

In this study, ionic liquid-based sugaring-out extraction was developed to separate lactic acid from the synthetic solution and actual lignocellulosic fermentation broth. Except for [EOHmim]BF4, the ILs with BF4− and OTF− anion can form aqueous two-phase system (ATPS) with the aid of saccharides. With the same kind of saccharides, the ATPS formation ability of ILs could be promoted by increasing the side-chain length of ILs in the order of [Hmim]BF4 ≈ [Bmim]BF4 ˃ [Emim]BF4 due to the decrease in ILs’ kosmotropicity. On the other hand, for the same type of ILs, an ATPS was formed more easily with glucose than with xylose. When IL concentration varied from 35% (w/w) to 40% (w/w) at a low glucose concentration of 15% (w/w), an interesting phase reversal was observed. When lactic acid was undissociated at pH 2.0, 51.8% LA and 92.3% [Bmim]BF4 were partitioned to the top phase, and 97.0% glucose to the bottom phase using an ATPS consisting of 25% (w/w) glucose and 45% (w/w) IL. The total recovery of LA would increase to 89.0% in three-stage sugaring-out extraction from synthetic solution. In three-stage sugaring-out extraction from the filtered and unfiltered fermentation broth obtained via simultaneous saccharification and co-fermentation (SSCF) of acid-pretreated corn stover by the microbial consortium, the total recovery of LA was 89.5% and 89.8%, respectively. Furthermore, the total removal ratio of cells and pigments from the unfiltered broth was 68.4% and 65.4%, respectively. The results support IL-based sugaring-out extraction as a potential method for the recovery of lactic acid from actual fermentation broth.Graphical

Highlights

Lactic acid (LA), an important platform chemical, is widely used in the food, pharmaceutical, and chemical industries, as a monomer of biodegradable and biocompatible polylactic acid (Ahmad et al 2020)
Glucose is more kosmotropic than xylose, and the ability to form an aqueous two-phase system (ATPS) of glucose is stronger than that of xylose
In this study, ionic liquid-based sugaring-out extraction was developed and investigated to separate lactic acid from synthetic solution and lignocellulose-derived fermentation broth

Summary

Introduction

Lactic acid (LA), an important platform chemical, is widely used in the food, pharmaceutical, and chemical industries, as a monomer of biodegradable and biocompatible polylactic acid (Ahmad et al 2020). Low-cost lignocellulosic feedstocks have received a lot of attention as an alternative carbon source for LA production because they are readily available, sustainable, and renewable (Isikgor and Becer 2015; Yuan et al 2018). Lignocellulose-derived lactic acid may produce more impurity components in broth than starch or sugar-based lactic acid production, such as lignin, protein, other organic acid, furan derivatives, and pigments. More complex processes and higher costs are required to separate LA from lignocellulose-derived broth (Xu et al 2018). Developing an efficient and cost-effective method for separating second-generation LA is both necessary and challenging. Economical and efficient recovery and purification processes for lignocellulosic lactic acid are desired

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