Ionic Liquid-Based Microwave-Assisted Extraction of Flavonoids from Bauhinia championii (Benth.) Benth.

Wei Xu,Lidian Chen,Yuqin Zhang,Ruilan Chen,Haiyin Zheng,Huang Li,Kedan Chu

doi:10.3390/molecules171214323

Wei Xu, Lidian Chen + Show 5 more

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https://doi.org/10.3390/molecules171214323

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Journal: Molecules	Publication Date: Dec 3, 2012
Citations: 69	License type: CC BY 4.0

Affiliation: Fujian University of Traditional Chinese Medicine

Abstract

An ionic liquids (IL)-based microwave-assisted approach for extraction and determination of flavonoids from Bauhinia championii (Benth.) Benth. was proposed for the first time. Several ILs with different cations and anions and the microwave-assisted extraction (MAE) conditions, including sample particle size, extraction time and liquid-solid ratio, were investigated. Two M 1-butyl-3-methylimidazolium bromide ([bmim] Br) solution with 0.80 M HCl was selected as the optimal solvent. Meanwhile the optimized conditions a ratio of liquid to material of 30:1, and the extraction for 10 min at 70 °C. Compared with conventional heat-reflux extraction (CHRE) and the regular MAE, IL-MAE exhibited a higher extraction yield and shorter extraction time (from 1.5 h to 10 min). The optimized extraction samples were analysed by LC-MS/MS. IL extracts of Bauhinia championii (Benth.) Benth consisted mainly of flavonoids, among which myricetin, quercetin and kaempferol, β-sitosterol, triacontane and hexacontane were identified. The study indicated that IL-MAE was an efficient and rapid method with simple sample preparation. LC-MS/MS was also used to determine the chemical composition of the ethyl acetate/MAE extract of Bauhinia championii (Benth.) Benth, and it maybe become a rapid method to determine the composition of new plant extracts.

Highlights

Bauhinia championii (Benth.)Benth., first recorded in the book Nanning Drug Chi, belongs to the bauhinia leguminosae family
This study investigated the potentiality of various ionic liquids (IL) with different cations and anions as microwave absorption media, as well as the microwave parameters of an IL-microwave-assisted extraction (MAE) method which was used to extract flavonoid from Bauhinia championii (Benth.) Benth
The obtained extract from the Ionic Liquid-Based Microwave-Assisted Extraction (IL-MAE) approach was analysed by LC-MS-MS

Summary

Introduction

Bauhinia championii (Benth.)Benth., first recorded in the book Nanning Drug Chi, belongs to the bauhinia leguminosae family. It is bitter and acerbic in taste, mild in nature. According to the records in China Shezu Medicine and investigations of medical statistics among the She ethnic minority group, many doctors prescribed this medicine for rheumatic pain. It could improve the symptomatic signs of RA, obviously reduce arthragia and swelling and had good clinical effects.

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