Abstract
BackgroundIn recent years, ionic liquids and enzymes have been widely used in the separation and extraction processes of natural products. Chlorogenic acid (CGA) has important biological and pharmacological activities. It is significant to develop a green and efficient method to extract GCA from Flos Lonicera japonica (FLJ) by integrating the advantages of the ionic liquids and enzymes.ResultsThe optimal type of enzyme and ionic liquid was screened. Pectinase in [C6mim] Br aqueous phase was demonstrated to be an ideal combination. The parameters including extraction time, extraction temperature, pH, enzyme amount, and IL concentration were optimized systematically. Scanning electronic microscopy of FLJ samples demonstrated that pectinase and ionic liquid disposal both obviously facilitated the extraction process by destroying the structure of cell wall. Circular dichroism spectroscopy showed that ionic liquid enhanced the activity of the pectinase by altering its secondary structure.ConclusionsCompared with previous reported methods, ionic liquid-based enzyme-assisted extraction of GCA from FLJ was proved to be efficient and practical, offering a higher yield in a shorter time. A novel process was proposed for the extraction of active component from natural resources.
Highlights
In recent years, ionic liquids and enzymes have been widely used in the separation and extraction processes of natural products
The optimized Ionic liquid‐based enzyme‐assisted extraction (ILEAE) method was investigated and the extraction mechanism by ILEAE was discussed by scanning electron microscopy and circular dichroism spectroscopy
Sieving of enzyme and ionic liquid type We considered the kinds of enzymes of literature commonly used in the enzymatic hydrolysis of the cell wall
Summary
Ionic liquids and enzymes have been widely used in the separation and extraction processes of natural products. Chlorogenic acid (CGA) has important biological and pharmacological activities. It is significant to develop a green and efficient method to extract GCA from Flos Lonicera japonica (FLJ) by integrating the advantages of the ionic liquids and enzymes. Chlorogenic acid (CGA) is the major bioactive components of FLJ, and had been reported to have various biological and pharmacological activities such as antioxidant, anti-inflammatory, and anti-carcinogenic activities (Wu et al 2012; Shin et al 2015). We come up with a method to use ionic liquid aqueous phase to enhance the penetrability of the solvent over the extraction process and utilize the characteristics of enzymatic hydrolysis on cell wall to enhance. The optimized ILEAE method was investigated and the extraction mechanism by ILEAE was discussed by scanning electron microscopy and circular dichroism spectroscopy
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
