Abstract
The patch-clamp whole cell recording was used to demonstrate activation of membrane conductance to K +, Cl − and cations induced by acetylcholine (ACh) in the isolated acinar cells of the guinea pig nasal gland. A small outward K + current at 0 mV and a large transient and sustained inward current at −90 mV were evoked by ACh and ACh-evoked reversal potential was about −3 mV nearly to Cl − equilibrium potential in 140 mM KCl in the pipette and physiological saline in the bath. The ionic substitutional experiments indicated that ACh-evoked inward currents were carried by both Cl − and cations. Both outward and inward currents evoked by ACh were almost completely abolished by removal of external Ca 2+ and mimicked those evoked by a calcium ionophore A23187. These findings indicate that ACh-evoked membrane conductances are mediated by an increase in intracellular Ca 2+.
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