Abstract

In air-saturated solutions freshly prepared barley and maize leaf slices take up ions in the light and in the dark at similar rates. During greening of etiolated leaves there is a small increase in the rate of this ion uptake, which is independent of photosynthesis. In anaerobic solution in the light the rate of ion uptake increases considerably during the first 4 h of greening from an initial low uncoupler insensitive rate to attain the same rate of energy-dependent uptake as observed in air (light or dark). This increase is independent of concomitant substrate synthesis by photosynthetic CO 2 fixation. It is concluded that in anaerobic solution in the light after approx. 4 h of greening sufficient photosynthetic energy is produced to saturate the ion uptake mechanism(s) so that no further increase in the rate of uptake is observed with greening beyond this point.

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