Ion transport in heart mitochondria. XIII. The effect of ethylenediaminetetraacetate on monovalent ion uptake

Abstract

1. Ethylenediaminetetraacetate (EDTA) markedly activates the accumulation of Na + and Li + and the swelling which accompanies the ion uptake by isolated heart mitochondria. This activation is reflected in the removal of limited amounts of endogenous Mg 2+ and extensive loss of K +. The removal of these cations requires the presence of Na +, a source of energy, and a permeant anion as well as EDTA. The effects of EDTA on the activation of Na + uptake and cation removal are duplicated by chelators with a high affinity for Mg 2+, but not by ethyleneglycol-bis-(β-aminoethylether)-N, N′-tetraacetic acid. Mg 2+ at concentrations 5 to 6 times less than EDTA prevents both activation of Na + uptake and cation removal. 2. EDTA does not appear to be bound by heart mitochondria. At neutral pH the chelator penetrates into the mitochondrial water volume to the same extent as sucrose and mannitol. At pH 8.1 where the removal of mitochondrial Mg 2+ by EDTA is more effective, EDTA penetrates virtually the entire water volume. This penetration requires the presence of a source of energy, a transported cation such as Na +, and a permeant anion. It appears possible that the oscillations in ion uptake and swelling observed in the presence of EDTA at pH 8.1 may be related to the presence of the chelator in the interior compartment under these conditions.