Abstract
AbstractThe response characteristics of several ions, usually considered as electroinactive, were investigated at the thin‐layer amperometric detector based on ion transfer across the interface of water/nitrobenzene solidified by polyvinyl chloride and agar. The influence of some factors, such as applied potential, flow rate of mobile phase, and organic solvent in mobile phase, was studied by flow injection analysis of cations (choline, acetylcholine, tetramethylammonium, tetrabutylammonium, and cesium) and anions (ClO, IO, and ReO). The detection limit of the flow injection amperometric detector is in the range of 4 to 40 ng of these ions. Reversed‐phase chromatographic separation of choline and acetylcholine with amperometric detection was demonstrated.
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