Abstract
The chloride ion selective electrode is used for a rapid, simple, and reliable determination of chloride ion in biological materials (blood serum, urine, fish, and plant tissues), food products (milk, beef extract, nutrient broth and orange, tomato, and grapefruit juices), soils, and waste water (industrial and municipal). The method consists of treating the samples with perchloric acid (pH 1) and potassium peroxydisulfate and determining the chloride content either by a calibration curve or by known addition or analyte addition, using the chloride ion selective electrode. Such sample treatment eliminates most of the interferences occurring in the samples, including iodide, complexing and reducing compounds, and macromolecular and surface-active species. The method is suitable for a wide range of chloride concentration, e.g., 5010 ppm Cl- in nutrient broth and 4890 ppm in beef extract and as low as 12 and 80 ppm in soil extracts.
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