Abstract
High performance liquid chromatography (HPLC) with isocratic ion‐pair reversed‐phase separation and UV detection at 220 nm is proposed for the analysis of six amine azo dye isomers prohibited under a German ban. The analysis is achieved on a C18 polaris column (5 µm, 250 mm × 4.6 mm ID) using a mixture of 20 mM phosphate buffer pH = 2.5, containing 5 mM 1‐hexanesulfonic acid, sodium salt and methanol (65:35). A flow rate of 1.0 mL min−1 and a column temperature of 25°C were employed. A total of 16 amines have been studied for limits of detection, relative standard deviations (RSD%) and linearity. The method gave excellent separation between isomers.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
