Ion-exchange properties of cell walls in chickpea cultivars with different sensitivities to salinity

Abstract

Ion-exchange properties of polymeric matrices were compared for cell wall preparations isolated from roots and shoots of two cultivars of Cicer arietinum L. (cvs. Bivanij and ILC 482) with different sensitivities to salinity. Irrespective of growth conditions, the cell walls contained four types of ionogenic groups: amino groups, carboxyl groups of uronic and hydroxycinnamic acids, and phenolic hydroxyl groups. Regardless of the salt concentration in the medium, the cells walls of different chickpea cultivars and from different organs of the same plant were similar in qualitative composition of ionogenic groups, although quantities of ionogenic groups per unit dry wt of cell walls varied depending on external and internal factors. Irrespective of the external medium salinity, the cation-exchange capacity of cell walls, expressed per unit dry wt, decreased in a sequence: stem > root ∼ bottom leaves > upper leaves. The volume of chickpea cell walls was found to vary depending on ionic composition and pH of the incubation medium. The results were analyzed in the context of cell wall involvement in responses of C. arietinum to elevated salinity.