Abstract
An ion-exchange chromatographic procedure for the analysis of iodothyronine mixtures containing iodotyrosines is described. Eight different iodothyronines are separated into five peaks and one shoulder, the order of their elution being related to the number and position of iodine substitution in the compounds. The resolution of monoiodotyrosine and diiodotyrosine from each other and from iodothyronines is excellent. Quantitation of iodoamino acids is carried out by the automatic analysis of the effluent based on the ceric-arsenite reaction. This procedure has been applied to the determination of the iodoamino acid distribution in hog thyroglobulin and to the analysis of photodegradation products of thyroxine and triiodothyronine.
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