Ion dependence of the eel taste response to amino acids

Abstract

The effects of changed ionic environments on the taste responses to amino acids were examined by recording the activity of the palatine nerve. The responses to glycine, l-proline and l-arginine dissolved in deionized water were greatly reduced after ions were removed from the surface of the palatine epithelium by treatment with 5 mM EDTA. The addition of various species of salts to amino acid solutions caused reversible recovery of the responses. The response to 0.1 mM glycine increased with an increase in salt concentration in amino acid solutions and reached saturation level. The effects of salts of divalent cations (CaCl 2 and MgCl 2) appeared at much lower concentration (10 −7 M) than those of salts of monovalent cations (NaCl and KCl) (10 −4 M), suggesting that cations support the taste response. All the organic cations examined, including those of large molecular weights (choline, Tris, bis-Tris, bis-Tris propane, tetraethylammonium, triethanolamine and d-glucosamine), also supported the taste responses. The results obtained led to a conclusion that not a specific cation but various species of cations can support the eel taste responses. It is suggested that the cations do not act as current carrying ions to depolarize the taste cells but that the binding of the cations to the receptor membrane plays an essential role in taste reception.