Ion channels and myogenesis in Duchenne muscular dystrophy: a first characterization in differentiating wild-type and dystrophic myocytes

Abstract

In skeletal muscle, several ion channels interact with the dystrophin complex, which is disrupted in Duchenne muscular dystrophy (DMD). However, little is known about the early involvement of ion channels in DMD, with particular focus to myogenesis. Here, we performed a pilot study to characterize the electrophysiological asset of myoblasts and myocytes by using two immortalized mouse satellite-derived cell lines: the wild-type 2B4 and the dystrophic SF1. For each cell line, Inward and outward currents were recorded at the proliferative state (myoblasts) and at different time points of differentiation (2, 4, 6 and 11 days) by whole-cell patch clamp.