Abstract

Planar lipid bilayer membranes were formed at the tip of glass patch clamp pipettes, and ion channels reconstituted by fusion of protein-containing vesicles with the bilayer. The advantages of the method over conventional bilayer techniques are numerous and include better current resolution, improved bilayer stability, compatibility with patch clamp apparatus and simple handling. Completely solvent-free lipid bilayers without any pretreatment can be prepared and only small amounts of materials are required.

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