Abstract

Scanning transmission ion microscopy (STIM) with energy loss contrast has been used to study the effect of ion microbeam irradiation on semi-thick animal tissue specimens. Excised, freeze substituted Drosophila melanogaster brains have been scanned with a 3 MeV proton beam of current 150 pA focused to a spot size of 5 μm for exposures between 0.15 and 2.22 mC/cm 2. Sample mass loss, volume loss and specimen deformation resulting from these irradiations have been observed with STIM using a 3 MeV proton beam current of ∼ 2000 ions/focused to a spot size of ∼ 0.5 μm. The similarity of the sample mass loss and volume loss curves suggests that volume losses are directly linked with the same beam specimen interactions that give rise to mass loss.

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