Abstract
The ability of cell-free extracts and participate fractions from Proteus mirabilis to accumulate silicate was found to be independent from previous adaptation of the cells to silicate. Preparations from silicate-adapted material gave 20–30% better results with respect to the amount of silicate accumulated, but responded more sensitively to minor alterations in the external phosphate concentration. The competitive effect of phosphate was found to occur in cell-free extracts as well as in the particulate preparations. The energy-requiring accumulation of silicate can be supported by either substrate-oxidation (NADH, succinate), or ATP cleavage. While silicate-fixation represents an unspecific ion accumulation process, the specific choice of the suitable membrane-bound ions depends on the ADP present in the system. The respiration-dependent but unspecific accumulation process and the highly specific ADP-ATP reaction combined seemed to act as a control mechanism, regulating the alternative stimulation and depression of either process.
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